This study aims to investigate the effect of sanctions on the banking system and its impact on the profitability of 22 Iranian Banks during years 2007-2014. The method of the study is feasible generalized least squares (FGLS). The result shows that the central bank and swift sanctions (directly) and blocking a large amount of oil by making stagflation situation (indirectly) has a negative impact on profitability ratios. Furthermore, banks sanctions by refusing letters of credit (LC) through banks of other countries has negative effects on both of return on assets and return on equity.